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目的 :探讨冬凌草甲素对 HL - 6 0细胞的生长抑制作用及其对细胞端粒酶活性的影响。方法 :应用噻唑蓝 (MTT)还原法检测细胞生长抑制率 ;流式细胞仪检测细胞凋亡及 bcl- 2基因的表达水平 ;应用端粒重复扩增 (TRAP)法检测端粒酶活性。结果 :5 nm ol/ L～ 2 5 nm ol/ L 的冬凌草甲素可诱导细胞凋亡 ,抑制细胞增生。在细胞生长受抑制过程中 ,端粒酶活性及 bcl- 2表达水平显著下降。结论 :冬凌草甲素可诱导急性白血病 HL- 6 0细胞凋亡、抑制细胞增生 ;其作用机制与端粒酶活性下降及 bcl- 2的表达水平下调有关 ,其中 bcl- 2的表达水平可能起重要的调节作用。
Objective: To investigate the inhibitory effect of oridonin on the growth of HL - 60 cells and its effect on telomerase activity. Methods: Cell growth inhibition rate was detected by MTT reduction assay. Cell apoptosis and bcl-2 gene expression were detected by flow cytometry. Telomerase activity was detected by telomeric repeat amplification (TRAP) assay. Results: Oridonin 5 nmol / L ~ 25 nm ol / L could induce cell apoptosis and inhibit cell proliferation. During the inhibition of cell growth, telomerase activity and bcl-2 expression decreased significantly. CONCLUSION: Oridonin can induce the apoptosis of HL-60 cells and inhibit the proliferation of HL-60 cells. The mechanism is related to the decrease of telomerase activity and the decrease of bcl-2 expression, and the expression level of bcl-2 may be Play an important regulatory role.