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目的研究细胞外环境低钙对培养心肌细胞损伤的影响机制。方法观察细胞外钙离子浓度为１．０ｍｍｏｌ／Ｌ、０．８ｍｍｏｌ／Ｌ、０．６ｍｍｏｌ／Ｌ、０．４ｍｍｏｌ／Ｌ、０．２ｍｍｏｌ／Ｌ时对原代培养心肌细胞膜流动性、细胞内游离钙和肌酸磷酸激酶（ＣＫ）的影响。结果钙离子浓度为０．６ｍｍｏｌ／Ｌ以下能明显引起膜流动性降低，细胞内游离钙增加，呈明显的量效关系。低钙能引起ＣＫ量增加，呈明显的量效关系。结论细胞外环境钙离子浓度降低能引起细胞内游离钙增加，进而使细胞损伤。
Objective To study the mechanism of extracellular low calcium on cardiomyocyte injury. Methods The extracellular calcium concentration of 1.0mmol / L, 0.8mmol / L, 0.6mmol / L, 0.4mmol / L, 0.2mmol / L of primary culture of myocardial cell membrane fluidity, intracellular free Effects of calcium and creatine phosphokinase (CK). Results When the concentration of calcium was below 0.6mmol / L, the membrane fluidity was obviously decreased and the intracellular free calcium was increased, showing a significant dose-response relationship. Low calcium can cause CK increased, showed a significant dose-effect relationship. Conclusion The decrease of calcium concentration in extracellular environment can lead to the increase of intracellular free calcium, leading to cell injury.