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目的观察齐墩果酸对高脂饮食肥胖大鼠的影响并初步探讨其作用机制。方法SD大鼠44只随机分为正常对照组、高脂饮食组、高脂饮食+齐墩果酸低[20mg/(kg·d)]、中[40mg/(kg·d)]、高[80mg/(kg·d)]剂量组。每天定时灌胃,记录进食量,每周定时称体重1次。8周末股动脉采血,测血清生化指标。结果给予高脂饮食后大鼠体重明显增加,血清瘦素(leptin)、抵抗素(resistin)、游离脂肪酸(FFA)和低密度脂蛋白(LDL)增加,高密度脂蛋白减少(HDL),血清谷丙转氨酶(GPT)和丙二醛(MDA)升高。高脂饮食大鼠给予齐墩果酸后,体重减轻,血清瘦素、FFA降低,MDA减少,总抗氧化能力(T-AOC)增高。结论齐墩果酸可明显抑制高脂饮食大鼠的体重增加。其作用可能是通过降低血清瘦素水平,改善体内脂肪代谢,提高机体的抗氧化能力实现的。
Objective To observe the effect of oleanolic acid on obesity rats with high fat diet and to explore its mechanism. Methods Forty-four SD rats were randomly divided into normal control group, high-fat diet group, high fat diet + oleanolic acid low [20mg / (kg · d)], medium [40mg / (kg · d) 80mg / (kg · d)] dose group. Gavage regularly every day, record the amount of food, weekly weighed 1 time. 8 weekend femoral artery blood, serum biochemical indicators. Results After the high-fat diet was given, the body weight of the rats increased significantly. The levels of serum leptin, resistin, free fatty acid (FFA) and low density lipoprotein (LDL), HDL, Elevated alanine aminotransferase (GPT) and malondialdehyde (MDA). After oleanolic acid administration in high-fat diet rats, weight loss, serum leptin, FFA decreased, MDA decreased, total antioxidant capacity (T-AOC) increased. Conclusion Oleanolic acid can obviously inhibit the body weight gain of high-fat diet rats. Its role may be achieved by lowering serum leptin levels, improving body fat metabolism, and improving the body’s antioxidant capacity.