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目的分析2型糖尿病(T2DM)患者谷胱甘肽催化反应体系改变情况,评价还原型谷胱甘肽治疗T2DM的效果。方法选择2014年10月至2015年6月确诊的T2DM患者86例(T2DM组)和健康人群86例(对照组),检测其血清过氧化氢(H_2O_2)、过氧化脂(LPO)含量及超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、GSH过氧化物酶(GSH-Px)和谷胱甘肽还原酶(GR)活性;将T2DM组患者随机分为GSH组(给予还原型GSH治疗)和非GSH组(给予B族维生素治疗),每组43例。治疗2周后比较两组的SOD活性、糖化血红蛋白(Hb Alc)含量、感觉神经传导速度(SCV)和尿蛋白排泄率(UAER)水平的变化情况。结果 T2DM组患者的H_2O_2和LPO含量分别为(3.66±0.81)nmol/L和(6.35±1.43)nmol/L,明显高于对照组的(2.23±0.24)nmol/L和(4.14±0.86)nmol/L,组间比较差异有统计学意义(P均<0.01);T2DM组患者的SOD、GSH、GSH-Px及GR活性分别为(41.36±4.72)nu/ml、(3.87±1.56)μg/L、(0.28±0.05)ng/L和(0.19±0.06)ng/L,显著低于对照组的(81.47±9.73)nu/ml、(4.46±1.93)μg/L、(0.36±0.09)ng/L和(0.27±0.09)ng/L,组间比较差异有统计学意义(P均<0.01);治疗2周后,GSH组患者的SOD活性、Hb Alc、SCV及UAER明显优于非GSH组(P<0.05,P<0.01)。结论 T2DM患者谷胱甘肽催化反应体系改变明显,给予还原型GSH治疗能有效提高T2DM患者的SOD活性,改善SCV和UAER水平。
Objective To analyze the changes of glutathione catalytic system in patients with type 2 diabetes mellitus (T2DM) and evaluate the effect of reduced glutathione on T2DM. Methods Totally 86 patients with T2DM (T2DM group) and 86 healthy people (control group) diagnosed from October 2014 to June 2015 were enrolled in this study. Serum levels of hydrogen peroxide (H 2 O 2) and lipid peroxide (LPO) (SOD), glutathione (GSH), GSH-peroxidase (GSH-Px) and glutathione reductase (GR) activity. The patients in T2DM group were randomly divided into GSH group Prototype GSH treatment) and non-GSH group (given B vitamin treatment), 43 cases in each group. The changes of SOD activity, HbAc, SCV and UAER were compared between the two groups after 2 weeks of treatment. Results The levels of H_2O_2 and LPO in patients with T2DM were (3.66 ± 0.81) nmol / L and (6.35 ± 1.43) nmol / L, respectively, which were significantly higher than those in the control group (2.23 ± 0.24 nmol / L and 4.14 ± 0.86 nmol (P <0.01). The activities of SOD, GSH, GSH-Px and GR in T2DM patients were (41.36 ± 4.72) nu / ml and (3.87 ± 1.56) μg / L, (0.28 ± 0.05) ng / L and (0.19 ± 0.06) ng / L, respectively, which were significantly lower than those in control group (81.47 ± 9.73) nu / ml, / L and (0.27 ± 0.09) ng / L respectively, there was significant difference between the two groups (P <0.01). After 2 weeks of treatment, the activities of SOD, Hb Alc, SCV and UAER in GSH group were significantly higher than those in non-GSH Group (P <0.05, P <0.01). Conclusions Glutathione-catalyzed reaction system in patients with T2DM changes significantly. Treatment with reduced GSH can effectively improve SOD activity and improve SCV and UAER in T2DM patients.