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目的调查黑龙江省逊克县火山熔岩地区蜱携带新发蜱传病原体及复合感染情况。方法 2015年4-6月采集活蜱样本257只,利用PCR方法分别扩增6种新发蜱传病原体的特异性核酸片段,通过基因测序进一步鉴定。结果共检出斑点热群立克次体、巴尔通体和嗜吞噬细胞无形体核酸阳性样本分别为86、5和11例,感染率分别为33.46%、1.94%和4.28%。伯氏疏螺旋体、巴贝西虫和埃立克体的DNA核酸检测结果均为阴性。森林革蜱、嗜群血蜱和全沟硬蜱体内共检出复合感染8例,感染率为3.11%,证实该地区森林革蜱中存在斑点热群立克次体及巴尔通体,嗜群血蜱和全沟硬蜱存在斑点热群立克次体及嗜吞噬细胞无形体复合感染的情况。结论应加强黑龙江省逊克县火山熔岩地区蜱的防控。
Objective To investigate the pathogen and complex infection of tick-borne ticks in volcanic lava area of Xunke County, Heilongjiang Province. Methods Totally 257 live tick samples were collected from April to June in 2015, and 6 kinds of specific nucleic acid fragments of pathogen of ticks were amplified respectively by PCR and further identified by gene sequencing. Results A total of 86, 5 and 11 positive specimens of rhabdovirus, Bartonella and phagocyte were detected respectively. The infection rates were 33.46%, 1.94% and 4.28% respectively. Borrelia burgdorferi, Babesia and Ehrlichia DNA nucleic acid test results were negative. Infected rate was 3.11%. The results showed that there were spotted fever group rickettsiae, Bartonella, Bartonella, Ticks and Ixodes iridiform ticks exist hot spots rickettsia and phagocytic phagocytosis of the compound infection. Conclusion Prevention and control of ticks in volcanic lava area of Xunke County, Heilongjiang Province should be strengthened.