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目的:初步筛选二陈汤化痰止咳的有效部位。方法:小鼠随机分为12组:生理盐水组、克立停组(DHS)、二陈汤5个提取部位的高、低剂量组(15,3.75 g.kg-1),各组分别ig给药,连续3 d,2次/d,末次给药后1 h,采用氨水引咳法观察不同提取部位对小鼠咳嗽次数的影响;小鼠随机分为12组:生理盐水组、沐舒坦组(AHOS)、二陈汤5个提取部位的高、低剂量组(15,3.75 g.kg-1),各组分别ig给药,连续3 d,2次/d,末次给药后30 min,采用酚红法观察不同提取部位对小鼠气管酚红分泌量的影响;豚鼠随机分为12组:生理盐水组、氨茶碱组(Ami)、二陈汤5个提取部位的高、低剂量组(8.32,2.08 g.kg-1),各组分别ig给药,连续3 d,2次/d,末次给药后1 h,采用组胺诱喘法观察不同提取部位对小鼠翻倒时间的影响。并通过上述方法对5个不同提取部位进行药效学筛选。结果:二陈汤挥发油部位、正丁醇部位和水部位可显著抑制小鼠咳嗽次数、增加小鼠酚红分泌量及延长豚鼠翻倒时间(P<0.01,P<0.05,),而石油醚部位、乙酸乙酯部位对此作用不显著。结论:初步排除石油醚部位、乙酸乙酯部位,并确定二陈汤治疗湿痰证的有效部位为挥发油部位、正丁醇部位和水部位。
Objective: To screen the effective part of Erchen Tang and Huatan Zhike. Methods: The mice were randomly divided into 12 groups: high and low dose groups (15, 3.75 g.kg-1) treated with Salviae Miltiorrhizae The mice were randomly divided into 12 groups: normal saline group, mucosolvan Group (AHOS) and Erchen Decoction (15,3.75 g.kg-1) were given in high and low dose groups. Each group were given ig administration for 3 days and 2 times / d, respectively. After the last administration 30 min. Phenol red method was used to observe the effect of different extraction sites on tracheal phenol red secretion of guinea pigs. Guinea pigs were randomly divided into 12 groups: saline, aminophylline group (Ami) and Erchen decoction, Low-dose group (8.32,2.08 g.kg-1), each group were given ig administration for 3 days, 2 times / d, 1 h after the last administration, histamine induction was used to observe the different extraction sites on mice The impact of falling over time. Through the above method, five different extraction sites were selected for pharmacodynamic analysis. Results: The contents of volatile oil, n-butanol and water in Erchen decoction could significantly inhibit the cough frequency, increase the phenol red secretion and prolong the guinea pig overturning time (P <0.01, P <0.05) Parts, ethyl acetate part of this effect is not significant. Conclusion: The petroleum ether fraction and ethyl acetate fraction were initially excluded and the effective fractions of Erchen Decoction for treating phlegm-dampness syndrome were volatile oil fraction, n-butanol fraction and water fraction.