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实验证实流产妇女血清中,39％患者含有抗滋养组织的抗体,而在正常早期妊娠仅有6％。在未分化和分化的滋养细胞中,用高滴定价的抗血清可确定有多种抗原。本研究应用间接免疫荧光法,目的在于制备一种抗人体滋养组织的抗血清,并研究绒毛和胎盘中抗原抗体反应。材料和方法:用妊娠6～7周流产的绒毛悬液免疫家兔3只,其中一兔无抗体形成,二兔抗体滴定价为1:64和1:128。免疫球蛋白用硫酸铵盐析,得蛋白含量6.8克％的试剂,其对抗原的免疫性用琼脂
Experiments confirmed that 39% of sera from aborting women contained anti-trophic antibodies, compared to only 6% of normal early pregnancies. In undifferentiated and differentiated trophoblastic cells, high titer of antisera can be used to identify multiple antigens. In this study, indirect immunofluorescence was used to prepare an antiserum against human nourishing tissue and to study antigen-antibody responses in villi and placenta. MATERIALS AND METHODS: Three rabbits were immunized with the fluffy abortion of 6 ~ 7 weeks gestation. One of the rabbits was free of antibody and the titer of the two rabbits was 1:64 and 1: 128 respectively. Immunoglobulins salted out with ammonium sulfate, the protein content of 6.8 g% of the reagents, the immunity of the antigen with agar