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[目的]探讨壳脂胶囊对非酒精性脂肪性肝炎(NASH)大鼠TNF-α、IL-6及GLUT-4水平的影响。[方法]SD大鼠40只,分为正常组、模型组、壳脂胶囊组和罗格列酮组,每组各10只。用高脂饲料诱导大鼠NASH模型,造模同时进行药物干预;连续给药8周后处死,苏木精-伊红染色、油红O染色观察肝组织病理,ELISA检测血清TNF-α、IL-6水平以及肝组织匀浆TNF-α、IL-6及GLUT-4含量。[结果]与模型组比较,壳脂胶囊组脂肪变性和炎症反应程度均有一定的减轻;壳脂胶囊能明显降低大鼠血清及肝组织TNF-α、IL-6水平(P<0.05或P<0.01);壳脂胶囊组大鼠肝组织GLUT-4含量均高于模型组(P<0.05)。[结论]壳脂胶囊能够改善NASH大鼠模型脂肪样变,调节GLUT-4的分泌,并能够显著降低TNF-α、IL-6分泌,对NASH起到防止作用。
[Objective] To investigate the effect of Hsiaoyin capsule on the levels of TNF-α, IL-6 and GLUT-4 in nonalcoholic steatohepatitis (NASH) rats. [Method] Forty SD rats were divided into normal group, model group, Hypocapsule capsule group and rosiglitazone group, with 10 rats in each group. The model of NASH was induced by high-fat diet and the model was established. At the same time, the rats were sacrificed 8 weeks after the continuous administration. The rats were sacrificed, hematoxylin-eosin staining and oil red O staining were performed to observe the pathological changes of liver. -6 levels and liver homogenate TNF-α, IL-6 and GLUT-4 content. [Results] Compared with the model group, the lipid degeneration and the degree of inflammatory reaction of the Hsiaoyin Capsule group were alleviated to some extent. The Hsiaozhi Capsule could significantly reduce the levels of TNF-α and IL-6 in the serum and liver tissue of rats (P <0.05 or P <0.01). The content of GLUT-4 in the liver tissue of HH group was higher than that of model group (P <0.05). [Conclusion] Shezhi Capsule can ameliorate the steatosis, regulate the secretion of GLUT-4 in NASH rat model, and can significantly reduce the secretion of TNF-α and IL-6 and play a preventive role in NASH.