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目的 探讨血管内皮生长因子 (VEGF)及其受体fms样酪氨酸受体 1(flt 1)和含插入区的激酶受体 (KDR)在子宫内膜癌血管生成中的作用及其与内膜癌分化程度的关系。方法 采用免疫组织化学及原位杂交方法对 2 3例子宫内膜癌及 6例正常绝经期子宫内膜中VEGF、flt 1、KDR蛋白质及其mRNA进行检测 ,并对少数病例行Western印迹分析 ,以检测VEGF亚型在内膜癌组织的分布 ,用内皮细胞标志Ⅷ因子标记内膜癌组织中的微血管密度。结果 VEGF、flt 1、KDR蛋白质及其mRNA主要分布在子宫内膜癌组织血管内皮细胞及癌细胞胞质内。VEGF蛋白质在中分化 (G2 )、低分化 (G3 )内膜癌血管内皮细胞及癌细胞上的表达高于高分化内膜癌 (G1)及正常绝经期子宫内膜 (P <0 0 5 ) ,VEGFmRNA在不同分化程度内膜癌组织的表达差异无显著性意义 (P >0 0 5 ) ,但均大于正常绝经期子宫内膜 (P <0 0 5 ) ;flt 1蛋白质及flt 1mRNA在G3 内膜癌血管内皮细胞的表达高于G1、G2 及正常绝经期子宫内膜 (P <0 0 5 ) ,在癌细胞的表达差异无显著性意义 (P >0 0 5 ) ,但均高于正常绝经期子宫内膜 (P <0 0 5 ) ;KDR蛋白质在子宫内膜癌组织血管内皮细胞及癌细胞上的表达较强 ,但不随分化程度发生变化 ,其mRNA在中分化 (G2 )、低分化 (G3 )内膜癌血管内皮?
OBJECTIVE: To investigate the role of vascular endothelial growth factor (VEGF) and its receptor fms-like tyrosine receptor 1 (flt 1) and its insertion-containing kinase receptor (KDR) in angiogenesis of endometrial carcinoma and its relationship with The relationship between the degree of differentiation of membrane cancer. Methods Immunohistochemistry and in situ hybridization were used to detect the protein and mRNA of VEGF, flt 1 and KDR in 23 cases of endometrial carcinoma and 6 cases of normal menopause endometrium. Western blotting analysis was performed on a few cases, To detect the distribution of VEGF subtypes in endometrial carcinoma, endothelial cell markers Ⅷ factor markers in endometrial cancer microvessel density. Results VEGF, flt 1, KDR protein and mRNA mainly distributed in the endometrial carcinoma vascular endothelial cells and cancer cells in the cytoplasm. The expression of VEGF protein in differentiated (G2) and poorly differentiated (G3) endometrial carcinoma cells was higher than that in highly differentiated endometrial carcinoma (G1) and normal menopause endometrium (P <0.05) , But there was no significant difference in the expression of VEGFmRNA in different differentiated endometrial carcinomas (P> 0.05), but they were both higher than those in normal endometrium (P <0 05). The expression of flt 1 protein and flt 1 mRNA in G3 The expression of vascular endothelial cells in endometrial carcinoma was higher than that in G1, G2 and normal endometrium (P <0.05), but no significant difference was found in cancer cells (P> 0.05) Normal endometrium of menopause (P <0 05); KDR protein expression in endometrial carcinoma vascular endothelial cells and cancer cells, but does not change with the degree of differentiation, the mRNA in the differentiation (G2) , Poorly differentiated (G3) endometrial cancer vascular endothelial?