制备了以电子媒介体聚中性红固定辣根过氧化物酶(HRP)的生物传感器,采用循环伏安法对该传感器的性能进行了研究。结果表明,辣根过氧化物酶在该电极上实现了稳定的直接电子转移反应,传感器在H2O2浓度为3.18×10-8～3.18×10-3mol/L的范围内具有良好的线性相关性,检出限为6.36×10-9mol/L。应用于啤酒中H2O2的测定,回收率为88.5%～98.6%。 A biosensor based on poly (neutral red) immobilized horseradish peroxidase (HRP) was prepared by electron mediator. The performance of the sensor was investigated by cyclic voltammetry. The results showed that horseradish peroxidase had a stable direct electron transfer reaction on the electrode. The sensor had good linearity in the range of 3.18 × 10-8 ~ 3.18 × 10-3mol / L H2O2, The detection limit was 6.36 × 10-9mol / L. Applied to the determination of H2O2 in beer, the recovery rate was 88.5% ~ 98.6%.