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目的:观察NKT细胞在实验性自身免疫性脑脊髓炎(EAE)小鼠脾脏和肝脏中所占百分比的变化特点,探讨NKT细胞在EAE模型中的免疫调节作用。方法:以MOG35-5521肽诱导C57BL/6小鼠建立EAE模型并进行临床评分。于发病高峰期处死小鼠,分离脾脏和肝脏淋巴细胞,采用免疫荧光染色和流式细胞术(FCM)分析,观察EAE小鼠与正常小鼠脾脏和肝脏中NKT细胞在全部淋巴细胞中所占百分率的变化。结果:在EAE小鼠不同器官中,NKT细胞占淋巴细胞的百分率均较正常小鼠减少。脾脏NKT细胞百分率(%)从正常组2.22±0.14下降到EAE模型组1.94±0.07(P<0.05),肝脏NKT细胞百分率(%)从正常组5.52±2.17下降到2.67±1.41(P<0.05)。结论:NKT细胞在EAE模型C57BL/6小鼠脾脏和肝脏中增殖受抑,提示EAE发病可能通过对NKT细胞数量的调节进而影响其对免疫应答的调节。
Objective: To observe the changes of percentage of NKT cells in the spleen and liver of experimental autoimmune encephalomyelitis (EAE) mice and to explore the immunomodulatory effect of NKT cells in EAE model. Methods: The C57BL / 6 mice were induced with MOG35-5521 peptide to establish EAE model and clinical score. The mice were sacrificed at the peak of the disease onset, and the spleen and liver lymphocytes were isolated. Immunofluorescence staining and flow cytometry (FCM) analysis were performed to observe the proportion of NKT cells in the spleen and liver between EAE and normal mice in all lymphocytes Percentage change. Results: In different organs of EAE mice, NKT cells accounted for the percentage of lymphocytes than normal mice. The percent of NKT cells in spleen decreased from 2.22 ± 0.14 in normal group to 1.94 ± 0.07 in EAE model group (P <0.05), while the percentage of NKT cells decreased from 5.52 ± 2.17 to 2.67 ± 1.41 in normal group (P <0.05) . CONCLUSION: The suppression of NKT cell proliferation in the spleen and liver of EAE model C57BL / 6 mice suggests that the pathogenesis of EAE may affect the regulation of immune response by regulating the number of NKT cells.