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运用体内试验和体外试验的方法，研究了草甘膦对大鼠肝微粒体酶活力的影响，并对其作用机理进行了初步探讨。结果表明，体内试验中，草甘膦能明显增加细胞色素Ｐ４５０含量，诱导氨基比林Ｎ－脱甲基酶（ＡＰＮＤ）和乙基吗啡Ｎ－脱甲基酶（ＥＭＮＤ）的活力；体外试验中，在３．３×１０－８～３．３×１０－４ｍｏｌ／Ｌ浓度范围内，未见草甘膦对Ｐ４５０含量有明显增加，而在２×１０－６ｍｏｌ／Ｌ和２．５×１０－５～２×１０－４ｍｏｌ／Ｌ浓度范围内可分别对ＡＰＮＤ和ＥＭＮＤ呈现诱导作用，在２×１０－８～２×１０－４ｍｏｌ／Ｌ浓度范围内，随草甘膦浓度增高，ＡＰＮＤ和ＥＭＮＤ活力均有上升趋势。初步观察结果，草甘膦在体内对肝微粒体酶诱导作用机理与其促进蛋白质合成作用有关。
The effects of glyphosate on the activity of liver microsomes in rats were studied by in vivo and in vitro methods. The mechanism of action was also discussed. The results showed that, in vivo, glyphosate significantly increased the content of cytochrome P450 and induced the viability of aminopyrine N-demethylase (APND) and ethyl morphine N-demethylase (EMND). In vitro experiments In the concentration range of 3.3 × 10-8 ~ 3.3 × 10-4mol / L, there was no significant increase of P450 content of glyphosate, but at 2 × 10-6mol / L and 2.5 × 10 In the concentration range of -5 ~ 2 × 10-4mol / L, the induction of APND and EMND could be induced respectively. With the concentration of glyphosate increasing in the range of 2 × 10-8 ~ 2 × 10-4mol / L, APND and EMND vitality has an upward trend. Preliminary observations, glyphosate in vivo mechanism of liver microsomal enzyme induction and its role in promoting protein synthesis.