论文部分内容阅读
目的研究中药复方促使T淋巴细胞增殖。方法从SD大鼠血液里分离淋巴细胞,进行体外培养。设正常细胞对照组、阳性药物对照组、转移因子组、复方中药高剂量组、中剂量组、低剂量组。培养1d后把药加入培养液中,继续培养2d,加Am-Blue细胞增殖与活性检测试剂,避光培养4h后,通过酶标仪检测,分析淋巴细胞的增值情况。结果中药复方高、中、低剂量组OD值均明显增高,与正常细胞组比较P<0.01;与阳性药物对照组比较,P<0.01;高剂量组与中、低剂量组比较P<0.01;转移因子组与正常细胞组比较P<0.01。结论中药复方能促使T淋巴细胞增殖,且与剂量有关。
Objective To study the effect of traditional Chinese medicine prescriptions on the proliferation of T lymphocytes. Methods Lymphocytes were isolated from the blood of SD rats and cultured in vitro. Set normal cell control group, positive drug control group, transfer factor group, compound high dose group, medium dose group, low dose group. After 1d culture, the drug was added to the culture medium, and the culture was continued for 2 days. Am-Blue cell proliferation and activity assay reagents were added. After 4 hours of light culture, the proliferation of lymphocytes was analyzed by a microplate reader. RESULTS: The OD values of the high, medium and low doses of the traditional Chinese medicine compound group were significantly higher, compared with the normal group (P<0.01), compared with the positive control group (P<0.01), and compared with the high dose group and the medium and low dose group (P<0.01). The transfer factor group was compared with the normal cell group P<0.01. Conclusion Chinese medicine compound can promote the proliferation of T lymphocytes, and is related to the dose.