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目的研究十字花科葶苈属植物光果葶苈(Drabanemorosa L.var.leiocarpa Lindl.)中莱菔硫烷的最佳提取工艺,为进一步研究开发此植物奠定基础。方法利用正交实验设计确定最佳提取工艺,考察因素包括缓冲溶液pH值,超声提取时间和提取温度。使用HPLC检测提取物中莱菔硫烷含量:采用Eclipse XDB-ⅡC18柱,以乙腈和水进行梯度洗脱;梯度洗脱条件:A泵为乙腈,B泵为水;30min内乙腈的浓度从20%升至50%;检测波长为254nm;室温。结果所考察的因素中,对光果葶苈中莱菔硫烷提取率影响程度为:缓冲溶液pH值>超声提取时间>提取温度,tris-HCl缓冲溶液的pH对提取率的影响最大;HPLC测定的线性范围为0.45~5.4μg(r=0.9968)。结论光果葶苈中莱菔硫烷最佳提取工艺为:超声提取30min,tris-HCl缓冲溶液pH值为5.5,提取温度30℃;HPLC测定光果葶苈中莱菔硫烷含量的方法快速、准确。
Objective To study the optimum extraction process of sulforaphane in the genus Dracaena spp. From Drabanemorosa L. var. Leiocarpa Lindl. It will lay the foundation for further research and development of this plant. Methods Orthogonal experimental design was used to determine the optimal extraction process. The factors investigated included pH value of buffer solution, extraction time and extraction temperature. The content of sulforaphane in the extract was detected by HPLC: gradient elution was carried out with acetonitrile and water using Eclipse XDB-II C18 column; gradient elution conditions: A pump was acetonitrile and B pump was water; the concentration of acetonitrile from 30% Rose to 50%; detection wavelength of 254nm; room temperature. Results Among the factors studied, the influence degree of sulforaphane on the extraction rate of sulforaphane in C. glauca was: pH value of buffer solution> ultrasonic extraction time> extraction temperature. The pH value of tris-HCl buffer solution had the strongest influence on the extraction yield. HPLC determination The linear range was 0.45 ~ 5.4μg (r = 0.9968). Conclusion The optimal extraction process of sulforaphane in the light harvesting is as follows: ultrasonic extraction for 30 min, tris-HCl buffer solution pH 5.5, extraction temperature 30 ℃; HPLC method for determination of sulforaphane content .