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目的基于蛋白芯片技术探讨丁氏溃结灌肠液对结肠炎大鼠肠道组织相关靶点的影响,阐释丁氏溃结灌肠液改善肠道炎症反应及肠纤维化的可能机制。方法大鼠随机分为阳性药组、模型组、正常组、丁氏溃结灌肠液组,5只/组。除正常组之外,其他3组均给予3.5%硫酸葡聚糖钠(dextran sulfate sodium,DSS)喂养,结肠炎造模成功后,阳性药组使用美沙拉秦灌肠,丁氏溃结灌肠液组使用丁氏溃结灌肠液灌肠,每天1次,连续4周,停药1 d后,取大鼠结肠黏膜组织,采用GSR-CAA-67抗体蛋白芯片技术检测各组大鼠肠道组织炎症反应及肠纤维化相关蛋白的变化,筛选出各组差异性表达的蛋白。结果通过蛋白芯片检测,发现共有8种有意义的差异蛋白,其中模型组和空白组对照组对比筛选出的8种蛋白包括IFN-γ、促红细胞生成素(erythropoietin,EPO)、TIMP-2、TIM-1、IL-6、TIMP-1、TNF-α、IL-22,差异均有统计学意义(P<0.05)。另一方面,丁氏溃结灌肠液与美沙拉秦均对IL-6、TIMP-1有明显的抑制作用(P<0.05),丁氏溃结灌肠液对TNF-α、IL-22有抑制作用(P<0.05),美沙拉秦组则没有相同作用(P>0.05)。结论丁氏溃结灌肠液具有多靶点的作用,可能通过改善肠道炎症反应及肠纤维化达到治疗溃疡性结肠炎的目的。
OBJECTIVE: To study the effect of Ding’s ulcer-enema on intestinal target related to colitis in rats based on protein chip technology and to explain the possible mechanism of Ding’s ulcer-enema to improve intestinal inflammation and intestinal fibrosis. Methods The rats were randomly divided into positive drug group, model group, normal group, Ding’s ulcerative enema group, 5 rats / group. In addition to the normal group, the other three groups were given dextran sulfate sodium (DSS) 3.5%, after the success of colitis modeling, the positive drug group using mesalamine enema, Ding ulceration enema group The intestinal mucosa tissue of rat was taken for 1 d after stopping for 1 d. The GSR-CAA-67 antibody protein chip technique was used to detect the intestinal inflammatory reaction in each group And the changes of intestinal fibrosis related proteins were screened out of the various groups of differentially expressed proteins. Results There were 8 kinds of proteins with significant differences detected by protein microarray. Among them, 8 proteins screened by contrast between model group and blank control group included IFN-γ, erythropoietin (EPO), TIMP-2, TIM-1, IL-6, TIMP-1, TNF-α, IL-22, the differences were statistically significant (P <0.05). On the other hand, both Ding’s ulcerative enema and mesalamine significantly inhibited IL-6 and TIMP-1 (P <0.05), while Ding’s ulcer enema inhibited TNF-α and IL-22 (P <0.05), while mesalamine group did not have the same effect (P> 0.05). Conclusions Ding’s ulcer enema has multi-target effect, and may achieve the purpose of treating ulcerative colitis by improving intestinal inflammation and intestinal fibrosis.