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目的探讨大鼠脊髓压迫性损伤(compressed spinal cord injury,CSCI)解压后表皮生长因子受体(phosphorylated epidermal growth factor receptor,pEGFR)、pAkt1的表达变化及其与神经功能、有髓神经纤维数量变化的相关性,为CSCI解压后治疗策略的制定和药物的研发提供实验基础。方法采用自行设计的方法制作SD大鼠CSCI模型,造模成功后解压。运用BBB(Basso Beattie Bresnahan)评分观察动物解压后神经功能的恢复情况;通过Luxol fast blue(LFB)染色检测解压后1、7、14、21 d有髓神经纤维数量变化;运用免疫荧光双标(Double-labeling immunoflurescence)、免疫印迹(western blotting,WB)检测pEGFR、pAkt1的表达变化。结果 CSCI解压后,BBB评分和有髓神经纤维数量随时间延长而逐渐增加;与此同时,pEGFR、pAkt1表达亦上调且与BBB评分、有髓神经纤维数量增加趋势一致。结论CSCI解压后,神经功能有一定改善、有髓神经纤维数量有一定增加,这些变化与pEGFR表达上调有关,提示EGFR的活化参与了CSCI解压后的内源性修复。
Objective To investigate the expression changes of pEGFR and pAkt1 after decompression of compressed spinal cord injury (CSCI) in rats and its relationship with the changes of neural function and the number of myelinated nerve fibers Relevance, provide experimental basis for the development of treatment strategy and drug development after the decompression of CSCI. Methods CS rat model of SD rats was made by self-designed method and decompressed after successful modeling. The BBB (Basso Beattie Bresnahan) score was used to observe the recovery of neurological function after decompression. The number of myelinated nerve fibers at 1,7,14,21 days after decompression was detected by Luxol fast blue (LFB) staining. Double-labeling immunoflurescence and Western blotting were used to detect the expression of pEGFR and pAkt1. Results After decompression of CSCI, the number of BBB and the number of myelinated nerve fibers increased gradually with time. Meanwhile, the expressions of pEGFR and pAkt1 were also up-regulated, and consistent with the increase of BBB score and the number of myelinated nerve fibers. Conclusion After decompression of CSCI, the neurological function is improved and the number of myelinated nerve fibers is increased. These changes are related to the upregulation of pEGFR, suggesting that EGFR activation is involved in the endogenous repair after decompression of CSCI.