论文部分内容阅读
目的:通过观察抑癌基因P16异常甲基化在子宫颈癌中的阳性率,探讨抑癌基因P16异常甲基化在子宫颈癌早期诊断中的临床意义。方法:分离提取71例慢性宫颈炎患者。76例CIN患者,36例宫颈癌患者标本中的DNA,采用巢式甲基化特异性PCR方法检测P16基因的甲基化状态。结果:在7例宫颈癌患者标本检测到了P16异常甲基化,阳性率为19.4%;在4例CIN癌患者标本中检测到了P16异常甲基化,阳性率为5.3%;健康时照者标本中P16基因无异常甲基化。结论:抑癌基因P16异常甲基化随宫颈瘤样病变的加重而阳性率增高,P16异常甲基化是宫颈发生的过程中的早期事件,对于宫颈癌的早期诊断具有重要意义。
Objective: To investigate the clinical significance of abnormal methylation of tumor suppressor gene P16 in the early diagnosis of cervical cancer by observing the positive rate of abnormal methylation of tumor suppressor gene P16 in cervical cancer. Methods: Seventy-one patients with chronic cervicitis were isolated and extracted. 76 cases of CIN patients, 36 cases of cervical cancer specimens of DNA, the use of nested methylation-specific PCR method to detect the methylation status of P16 gene. Results: The abnormal methylation of P16 was detected in 7 cases of cervical cancer, the positive rate was 19.4%. The abnormal methylation of P16 was detected in 4 cases of CIN. The positive rate was 5.3% P16 gene in abnormal methylation. Conclusion: The abnormal methylation of tumor suppressor gene P16 is associated with the increase of cervical neoplasia. However, the abnormal methylation of P16 is an early event in cervical carcinogenesis, which is of great significance for the early diagnosis of cervical cancer.