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目的：评价溴乙锭荧光法检测食用油烟、香烟烟气、蚊香烟气颗粒提取物、氧化型染发剂、硝基苯类化合物的ＤＮＡ交联作用。方法：ＤＮＡ交联溴乙锭荧光分析法（ＥＦＡ）基于双链间交联所致的ＤＮＡ在变性条件下不解链，与溴乙锭结合可产生较强荧光，而正常ＤＮＡ变性后形成的单链ＤＮＡ无此现象的原理对ＤＮＡ交联进行测定。结果：食用油烟和香烟烟气颗粒提取物、氧化型染发剂具有致小牛胸腺ＤＮＡ交联形成的作用，具有遗传毒性的蚊香烟气颗粒提取物和２，４－二硝基甲苯和间二硝基苯等硝基苯化合物未观察到致ＤＮＡ交联作用。结论：ＤＮＡ交联溴乙锭荧光方法灵敏、快速、简便，适用于对环境污染物ＤＮＡ损伤机制的研究。
OBJECTIVE: To evaluate the DNA cross-linking effect of ethidium bromide using ethidium bromide fluorescence spectrometry for the detection of edible fume, cigarette smoke, particulate extract of mosquito flue gas, oxidative hair dye and nitrobenzene compounds. Methods: DNA cross-linking Ethidium bromide fluorescence spectrometry (EFA) based on cross-linked DNA cross-linked DNA under denaturing conditions do not melt, combined with the ethidium bromide can produce strong fluorescence, and normal DNA denatured formed The principle of single-stranded DNA without this phenomenon was determined by DNA cross-linking. Results: Edible fume and cigarette smoke particulate extract, oxidative hair dye had the effect of cross-linking induced calf thymus DNA, genotoxic mosquito cigarette smoke particulate extract and 2,4-dinitrotoluene Nitrobenzene compounds such as dinitrobenzene were not observed to cause DNA cross-linking. Conclusion: The method of DNA cross-linking by ethidium bromide is sensitive, rapid and simple, and it is suitable for studying the DNA damage mechanism of environmental pollutants.