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Nanocrystalline TiO2 thin films were successfully prepared by a new "water-cooked"method on both conductive glass substrates and flexible substrates at low temperature.Dye-sensitized solar cells based on these films have exhibited high overall light-to-electricity conversion efficiencies of 4.67 % and 1.94 % on conductive glass substrate and flexible substrate,respectively, under the illumination at 100 mW/cm2.