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目的通过检测宫颈上皮内瘤样病变及宫颈癌组织中HPV16、HPV18的感染情况,探讨其与宫颈癌及癌前病变的关系。方法应用荧光定量PCR(FQ-PCR)技术对宫颈组织进行HPV DNA定性检测,以光镜下病理学诊断作为CIN分级标准。结果268例宫颈组织中HPV16、HPV18的总检出率分别为44.78%(120/268)、8.96%(24/268),CINⅡ～Ⅲ级和宫颈鳞癌组织中HPV16的检出率明显高于HPV18(P﹤0.05)。HPV16或HPV18以单一型别感染为主,5例存在双重感染(P﹤0.01)。HPV16的检出率随CINⅠ级、CINⅡ～Ⅲ级和宫颈鳞癌病变程度的加重而显著增高(P﹤0.05)。HPV18在不同宫颈病变组织中的检出率无显著性差异。结论HPV16感染可能与宫颈癌及其癌前病变的发生发展密切相关,其定性检测对宫颈癌可以起到一定的预测作用。
Objective To explore the relationship between cervical intraepithelial neoplasia (HPV) and cervical precancerous lesions by detecting the infection of HPV16 and HPV18 in cervical intraepithelial neoplasia and cervical cancer. Methods The qualitative and quantitative HPV DNA detection of cervical tissue was performed by fluorescence quantitative PCR (FQ-PCR). The pathological diagnosis of cervical lesions was taken as the CIN grading standard. Results The total positive rates of HPV16 and HPV18 in 268 cervical tissues were 44.78% (120/268) and 8.96% (24/268), respectively. The positive rates of HPV16 in CINⅡ ~ Ⅲ and cervical squamous cell carcinoma were significantly higher than those in cervical squamous cell carcinoma HPV18 (P <0.05). HPV16 or HPV18 were predominantly single type infection, and 5 cases were double infection (P <0.01). The detection rate of HPV16 was significantly increased with the severity of CINⅠ, CINⅡ ~ Ⅲ and cervical squamous cell carcinoma (P <0.05). The detection rate of HPV18 in different cervical lesions showed no significant difference. Conclusion HPV16 infection may be closely related to the occurrence and development of cervical cancer and its precancerous lesions, and its qualitative detection may play a certain role in predicting cervical cancer.