采用组织学完整的病人标本，以裸小鼠肝被膜下植入方法，从３０例人肝癌标本中筛选出一株人肝癌高转移瘤株，在裸小鼠体内成功地建立了人肝癌高转移模型ＬＣＩ－Ｄ２０。目前该瘤株在裸鼠体内生长１年，传代１６次，其移植生长率与自发转移率达１００％，表现为区域侵犯、肝内、淋巴结和肺转移、腹腔种植与血性腹水，并保持分泌甲胎蛋白的特性。组织病理学和电镜观察，流式细胞仪ＤＮＡ相对含量分析及染色体核型分析结果表明，移植瘤细胞与来源人肝癌细胞相似。本模型为原位移植模型，移植瘤的生长与转移，完全模拟了人肝癌的自然过程，为研究人肝癌转移机制和抗转移治疗提供了一个理想模型。 Using a histologically intact patient specimen and subcutaneous implantation of nude mice, a human high hepatoma metastasis strain was screened from 30 human hepatoma specimens, and a high metastasis of human hepatoma was successfully established in nude mice. Model LCI-D20. At present, the tumor strain grows for 1 year in nude mice and is passaged 16 times. Its transplantation growth rate and spontaneous metastasis rate reach 100%, manifested as regional invasion, intrahepatic, lymph node and lung metastasis, abdominal planting and bloody ascites, and maintaining secretion. The characteristics of alpha-fetoprotein. Histopathological and electron microscopy observations, relative flow cytometric DNA content analysis and karyotype analysis showed that the transplanted tumor cells were similar to human liver cancer cells. This model is an orthotopic transplantation model. The growth and metastasis of transplanted tumors completely simulates the natural process of human liver cancer, providing an ideal model for studying the mechanism of metastasis of human liver cancer and anti-metastasis therapy.