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目的 探讨 β 内啡肽及阿片 μ受体在脑内移植脑源性神经营养因子 (BDNF)载体细胞治疗新生大鼠低氧缺血性脑损伤 (HIBI)中的作用。方法 7d龄新生大鼠随机分为HIBI+BDNF组 (A)、HIBI +BDNF +DAMGO组 (B)、单纯HIBI组 (C)和假手术组 (D)。左侧颈总动脉结扎联合低氧吸入形成新生大鼠HIBI,伤后立即脑内植入BDNF载体细胞 (A、B)或空白细胞 (C) ,植入后延髓池内注射阿片 μ受体激动剂DAMGO (0 2 μg ,B) ,观察处理后 0、 1、 3d左侧皮层和海马 β 内啡肽免疫活性物质 (ir β ΕΡ)含量的变化及处理后 1d脑含水量、丙二醛 (MDA)含量和细胞凋亡情况。结果 (1)ir β ΕΡ含量 ,左侧皮层A组显著低于C组 (0d) ,B、C组显著高于D组 (0、 1d) ,海马A、B、C组均显著高于D组 (0d) ,C组显著高于A组或D组 (1d)。 (2 )左脑含水量C组显著高于A、B或D组 ,MDA水平A、B、C组均显著高于D组 ,A组显著低于B或C组。 (3)细胞凋亡百分率 ,左侧皮层B、C组显著高于D组 ,C组显著高于A或B组 ,A组显著低于B组 ;海马A、B、C组显著高于D组 ,A组显著低于C组。结论 β 内啡肽参与HIBI病理生理过程。脑内植入BDNF载体细胞影响HIBI的作用环节之一 ,是抑制了 β 内啡肽与其特异性阿片 μ受体相结合对HIBI所形成的效应
Objective To investigate the role of β-endorphin and opioid μ receptor in transplanted brain-derived neurotrophic factor (BDNF) vector cells in neonatal rats with hypoxic-ischemic brain damage (HIBI). Methods 7d-old neonatal rats were randomly divided into three groups: HIBI + BDNF group (A), HIBI + BDNF + DAMGO group (B), HIBI group alone (C) and sham operation group (D). The left common carotid artery ligation combined with hypoxia inhalation formed neonatal rat HIBI. Immediately after injury, BDNF vector cells (A, B) or blank cells (C) were implanted in the brain. After injection, opioid μ receptor agonist DAMGO (0 2 μg, B). The levels of β-endorphin immunoreactive substance (ir β ΕΡ) in the left cortex and hippocampus at 0, 1 and 3 days after treatment were observed. The changes of brain water content, ) Content and apoptosis. Results: (1) The content of irβEEP in left cortex was significantly lower in group A than that in group C (0d), while in group B and C was significantly higher than that in group D (0 and 1d) Group (0d), group C was significantly higher than group A or group D (1d). (2) The left brain water content in group C was significantly higher than that in group A, B or D, the levels of MDA in group A, B and C were significantly higher than those in group D, and the levels in group A were significantly lower than those in group B or C. (3) The percentage of apoptosis in the left cortex was significantly higher in group B and C than in group D, and in group C was significantly higher than that in group A or B, and in group A was significantly lower than that in group B; Group A, Group A was significantly lower than Group C Conclusion β-endorphin is involved in HIBI pathophysiological process. Implantation of BDNF-bearing cells into the brain affects one aspect of HIBI, one of which is the inhibition of the effect of binding of beta-endorphin to its specific opioid μ receptor on HIBI