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AIM: To study the signal roles of protein kinase C (PKC) and protein kinase A (PKA) in the influence of interferon-γ(IFN-γ) on proliferation and collagen synthesis of flbroblasts derived from hypertrophic scar (HS-FB) and normal skin (NS-FB). METHODS: HS-FB and NS-FB were cultured and passaged in Dulbecco’s modified Eagle’s medium (DMEM). Activity of PKC and PKA were assayed by transferring phosphorus (32P) into substrate after treatment with IFN-γ1000 kU/L at 10, 30, 60, and 120 min. Cell proliferation was determined with MTT assay. The collagen synthesis was measured with [3H]proline incorporation and Type Ⅲ pre-collagen was determined with radioimmunoassay. RESULTS: After exposure to IFN-γ1000 kU/L for 30 min, PKC activity of HS-FB and NS-FB increased from 2.57±0.14 and 2.13±0.12 nmol·min-1·g-1 of control to 3.75±0.19 and 3.36±0.16 nmol·min-1·g-1 respectively (P<0.05). After exposure to IFN-y 1000 kU/L for 60 and 120 min, PKA activities of HS-FB increased gradually from 0.82±0.04 nmol·m
AIM: To study the signal roles of protein kinase C (PKC) and protein kinase A (PKA) in the influence of interferon-γ (IFN-γ) on proliferation and collagen synthesis of flbroblasts derived from hypertrophic scar METHODS: HS-FB and NS-FB were cultured and passaged in Dulbecco’s modified Eagle’s medium (DMEM). Activity of PKC and PKA were assayed by transferring phosphorus (32P) into substrate after treatment with IFN- The collagen synthesis was measured with [3H] proline incorporation and Type III pre-collagen was determined with radioimmunoassay. RESULTS: After exposure to PKC activity of HS-FB and NS-FB increased from 2.57 ± 0.14 and 2.13 ± 0.12 nmol · min-1 · g-1 of control to 3.75 ± 0.19 and 3.36 ± 0.16 nmol · IFN-γ1000 kU / L for 30 min min-1 · g-1 respectively (P <0.05). After exposure to IFN-γ 1000 kU / L for 60 and 120 min, PKA activities of HS- m 0.82 ± 0.04 nmol · m