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【目的】通过病理学方法研究胶原酶对大鼠脊神经背根神经节的损伤情况。【方法】57只健康的Sprague-Dawley雄性大鼠分为正常对照组;急性胶原酶手术组、亚急性胶原酶手术组和慢性胶原酶手术组;急性生理盐水手术组、亚急性生理盐水手术组和慢性生理盐水手术组。暴露大鼠左侧腰5背根神经节,并在各胶原酶手术组局部滴注胶原酶1mL(300U),各生理盐水手术组局部滴注生理盐水1mL。分别于注药后1h、1周、30d处死动物并取包含背根神经节的一段神经做病理学检测。【结果】光镜下各组背根神经节的细胞数、胞膜、胞浆、胞核形态和细胞间神经纤维、血管相比较均无明显变化和差别。透射电镜下各组背根神经节的细胞数、细胞大体形态、胞膜和节内神经纤维相比较均无明显变化和差别,但胶原酶手术组背根神经节细胞胞内细胞器超微结构与正常对照组和相应生理盐水手术组比较有差别,表现为核仁部分偏向一侧、线粒体大量肿胀,部分嵴断裂和空泡形成。光镜病理和透射电镜均未见节细胞成群细胞坏死、细胞膜早期破裂和凋亡小体形成。【结论】临床应用的胶原酶化学髓核溶解术的胶原酶治疗浓度对大鼠脊神经背根神经节细胞有损伤作用。在临床工作中一定要慎重使用胶原酶的用药剂量和浓度,才能提高胶原酶应用的安全性。
【Objective】 To study the damage of collagenase to dorsal root ganglion of spinal dorsal root ganglia in rats by pathology. 【Methods】 Fifty-seven healthy male Sprague-Dawley rats were divided into normal control group, acute collagenase group, subacute collagenase group and chronic collagenase group, acute saline group, subacute saline group And chronic saline surgery group. The left dorsal root ganglion (DRG) of the left lumbar 5 was exposed, and 1 mL collagenase (300 U) was instilled locally in each collagenase group. Local saline instillation group was given 1 mL normal saline. Animals were sacrificed at 1h, 1 week and 30d after injection, respectively, and a section of nerve including dorsal root ganglion was taken for pathological examination. 【Result】 There was no significant difference and difference in cell number, cell membrane, cytoplasm, nuclear morphology and intercellular nerve fibers and blood vessels in each group of dorsal root ganglion under light microscope. There were no significant differences and differences in the number of cells, the general morphology of cells, the nerve fibers between the membrane and the nodules in each group under the transmission electron microscope. However, the ultrastructure of intracellular organelles in the dorsal root ganglion There were differences between the normal control group and the corresponding saline surgery group, which showed that the nucleolus part was biased towards one side, the mitochondria swelled extensively, part of the crest ruptured and the vacuole formed. No pathological necrosis of ganglion cells, early rupture of cell membrane and formation of apoptotic bodies were observed by light microscopy and transmission electron microscopy. 【Conclusion】 The clinical application of collagenase chemical nucleus pulposus collagenase treatment concentration on rat dorsal root ganglion cells injury. In clinical work must be careful use of collagenase dose and concentration, in order to improve the safety of collagenase application.