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目的:建立RP-HPLC法同时测定湿毒清胶囊中丹酚酸B及黄芩苷的含量。方法:Agilent SB-C_(18)柱,流动相:A相为甲醇,B相为3.2%冰醋酸水溶液,梯度洗脱流速1.0 ml·min~(-1),柱温:30℃,检测波长为286 nm。结果:丹酚酸B、黄芩苷的线性范围分别为0.08～0.71,0.46～11.64μg。回收率分别为97.5%,97.3%;RSD 0.5%,1.6%(n=5)。结论:方法简便可靠,结果稳定,重复性好,可用于湿毒清胶囊中丹酚酸B及黄芩苷的含量测定。
Objective: To establish an RP-HPLC method for simultaneous determination of salvianolic acid B and baicalin in Huduqing capsule. Method: Agilent SB-C_(18) column, mobile phase: A phase is methanol, B phase is 3.2% aqueous acetic acid, gradient elution flow rate 1.0 ml·min -1, column temperature: 30°C, detection wavelength It is 286 nm. Results: The linear ranges of salvianolic acid B and baicalin were 0.08-0.71 and 0.46-11.64 μg, respectively. The recoveries were 97.5% and 97.3%, respectively; RSD 0.5%, 1.6% (n=5). Conclusion: The method is simple, reliable, stable and reproducible. It can be used for the determination of salvianolic acid B and baicalin in Hudu Duqing Capsule.