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目的:探讨小鼠肝癌细胞H22在肿瘤淋巴道转移研究中的应用。方法:将小鼠肝癌细胞株H22分别接种于Km小鼠左腹股沟部(A组)和左后肢爪垫皮下(B组),于接种后分批处死小鼠。H-E染色观察A组移植瘤及B组腘淋巴结、腹股沟淋巴结;透射电镜下观察A组移植瘤内毛细淋巴管超微结构。结果:A组小鼠移植瘤周边区可见毛细淋巴管,其内皮细胞亚细胞结构发生改变。B组淋巴结发生癌转移的概率和程度与接种细胞数相关。结论:小鼠肝癌(H22)模型可作为一种重要的肿瘤移植性模型而在肿瘤的淋巴道转移研究中发挥作用。
Objective: To investigate the application of mouse hepatocellular carcinoma cell line H22 in lymphatic metastasis of tumor. Methods: Mouse hepatoma cell line H22 was inoculated into the left groin (group A) and the left hindlimb claw pad (group B) respectively in Km mice. The mice were sacrificed in batches after inoculation. H-E staining was used to observe the transplanted tumor in group A and lymph node and inguinal lymph node in group B. The ultrastructure of capillary lymphatic vessels in group A was observed under transmission electron microscope. Results: Capillary lymphatic vessels were observed in the periphery of the transplanted tumor in group A, and the endothelial cell subcellular structure was changed. The probability and extent of cancer metastasis in group B lymph nodes correlated with the number of inoculated cells. Conclusions: Mouse hepatocellular carcinoma (H22) model can play an important role in the study of lymphatic metastasis in tumor as an important model of tumor transplantation.