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目的 :测定鼻咽癌患者血清中活性氧 (ROS)、超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH PX)的活性水平 ,以探讨鼻咽癌患者体内自由基代谢状态及其在鼻咽癌发生、发展中的作用。方法 :分别采用Fenton反应显色法 ,黄嘌呤氧化酶法和二硫代二硝基苯甲酸法对 4 0例鼻咽癌患者 (鼻咽癌组 )和 2 0例健康体检者 (对照组 )血清中ROS、SOD和GSH PX水平进行检测。结果 :鼻咽癌组血清中ROS活性 [(11 32 7.835±4 83.777) /(U·ml-1) ]显著高于对照组 [(10 2 6 2 .712± 5 2 5 .2 87) /(U·ml-1) ](P <0 .0 5 ) ,SOD和GSH PX活性[(76 .6 19± 2 2 .2 83) /(NU·ml-1) ,(98.6 5 3± 4 6 .374 ) /AU ]显著低于对照组 [(15 4 .6 0 3± 2 7.2 4 1) /(NU·ml-1) ,(30 7.872± 116 .2 75 ) /AU](P <0 .0 5 ) ;不同病理类型鼻咽癌患者血清中ROS、SOD和GSH PX活性间的差异均无显著性意义 (P >0 .0 5 )。结论 :鼻咽癌患者体内自由基代谢紊乱 ,抗氧化损伤能力显著下降 ,给予抗氧化治疗有利于抑制鼻咽癌的发展
OBJECTIVE: To determine the levels of reactive oxygen species (ROS), superoxide dismutase (SOD) and glutathione peroxidase (GSH PX) in the serum of patients with nasopharyngeal carcinoma (NPC) to explore the effects of free radical metabolism State and its role in the occurrence and development of nasopharyngeal carcinoma. Methods: 40 cases of nasopharyngeal carcinoma (nasopharyngeal carcinoma) and 20 cases of healthy subjects (control group) were treated with Fenton reaction chromogenic method, xanthine oxidase method and dithiobisnitrobenzoic acid method, respectively. Serum ROS, SOD and GSH PX levels were detected. Results: The activity of ROS in the serum of nasopharyngeal carcinoma group was significantly higher than that of the control group [(11 32 7.835 ± 4 83.777) / (U · ml -1)] [(10 2 6 2 .712 ± 5 2 5 .2 87) / (P <0. 05), SOD and GSH PX activities [(76.66 ± 22.2.283) / (NU · ml-1), (98.6 ± 3.4) 6. 374) / AU] was significantly lower than that of the control group [(15 4 .6 ± 3 7.22 1) / (NU · ml-1), (30 7.872 ± 116.275) / AU] 0. 05). There was no significant difference in the serum levels of ROS, SOD and GSH PX between patients with different pathological types of NPC (P> 0.05). Conclusion: The metabolism of free radical in patients with nasopharyngeal carcinoma is disordered and the ability of anti-oxidative injury is significantly decreased. Anti-oxidative treatment is beneficial to inhibit the development of nasopharyngeal carcinoma