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综述了传质模型的内容和其在下游分离纯化中的应用以及结合高通量筛选技术对传质模型研究方法的优化,重点介绍了传质模型包括吸附等温线模型和吸附动力学模型在亲和层析介质动态结合载量预测中的应用。与传统下游分离纯化工艺研发相比,采用传质模型,一方面能全面评估实验条件对层析工艺开发的影响,给工艺开发及优化提供新的思路,另一方面也能减少工艺开发时间和耗材用量达到降低工艺开发成本的目的。同时,高通量筛选技术在层析工艺开发中的大量推广也逐渐改变传统工艺开发流程,大大降低工艺开发成本。大量研究表明。通过研究吸附等温线和吸附动力学模型,能够对层析介质的静态吸附能力以及动力学行为进行描述,准确预测亲和层析中产品的穿透曲线和亲和层析介质的动态结合载量,与实验室规模层析柱上实验结果一致性好,有望广泛应用于下游纯化工艺参数的优化和探索中。
The content of mass transfer model and its application in downstream purification and purification are reviewed. The optimization of mass transfer model research with high-throughput screening technology is reviewed. Mass transfer models including adsorption isotherm model and adsorption kinetic model are discussed in detail. And the application of chromatographic medium in dynamic loading prediction. Compared with the traditional downstream separation and purification process development, mass transfer model, on the one hand can fully evaluate the experimental conditions on the chromatographic process development, to provide new ideas for process development and optimization, on the other hand can also reduce process development time and Consumption of materials to achieve the purpose of reducing process development costs. At the same time, the large-scale promotion of high-throughput screening technology in the development of chromatographic process also gradually changes the traditional process development process and greatly reduces the process development cost. A large number of studies have shown. By studying the adsorption isotherm and adsorption kinetics model, the static adsorption capacity and kinetic behavior of the chromatographic medium can be described, and the breakthrough curves of the products in the affinity chromatography and the dynamic binding capacity of the affinity chromatography medium can be accurately predicted. It is expected to be widely used in the optimization and exploration of downstream purification process parameters in good agreement with the experimental results on a laboratory scale column.