作者用破伤风类毒素或重组破伤风毒素（TT）C片段多肽及由编码TT C片段质粒制备的DNA疫苗免疫雌性BALB/c小鼠3次,每次间隔两周。分别于每次注射后1周采血,以ELISA测定血清抗TT抗体滴度。另外,从免疫小鼠脾中分离单核细胞与C片段多肽一起培育,5天后加入[3~H]胸苷,测定T The authors immunized female BALB / c mice three times with a tetanus toxoid or recombinant tetanus toxin (TT) C fragment polypeptide and a DNA vaccine prepared from a plasmid encoding the TT C fragment for two weeks each. Blood samples were collected one week after each injection, and serum anti-TT antibody titers were measured by ELISA. In addition, mononuclear cells were isolated from the spleen of immunized mice and incubated with the C-segment polypeptide, [3 H] thymidine was added 5 days later, T