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目的评价重组腺病毒介导的低氧诱导因子基因导入是否具有促进SD大鼠骨组织工程的血管化的作用。方法通过重组低氧诱导因子腺病毒载体转染SD大鼠的骨髓基质细胞,然后将含有目的基因种子细胞与骨组织工程的支架材料藻酸钙复合成可注射性组织工程骨植入裸鼠背部皮下,应用Masson染色和SP免疫组化法观察异位成骨能力,检测构建组织的微血管密度计数(MVD)。结果动物实验显示,实验组新骨形成和新生血管形成良好,实验组的微血管化密度显著地高于对照组。结论低氧诱导因子基因导入有利于组织工程骨的新骨形成和再血管化。
Objective To evaluate whether recombinant adenovirus-mediated hypoxia inducible factor gene transfer can promote the vascularization of bone tissue in SD rats. Methods Transfected the bone marrow stromal cells of SD rats by recombined hypoxia inducible factor adenovirus vector, and then composite the calcium alginate, a scaffold material containing the target gene and bone tissue engineering, into injectable tissue engineering bone and implanting it into the back of nude mice Under the skin, the ectopic osteogenesis ability was observed by Masson staining and SP immunohistochemistry, and the microvessel density (MVD) of the constructed tissue was measured. Results Animal experiments showed that the formation of new bone and neovascularization were good in the experimental group, and the microvessel density in the experimental group was significantly higher than that in the control group. Conclusion Introduction of hypoxia inducible factor gene facilitates the formation of new bone and revascularization of tissue engineered bone.