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[目的]研究丛枝菌根真菌对Cu、Pb处理下旱稻氧化胁迫的影响。[方法]采用盆栽实验的方法,以旱稻为研究材料,研究了丛枝菌根真菌对不同土壤Cu浓度(0、100、200 mg/kg)、Pb浓度(0、300、600 mg/kg)处理下旱稻地上部分生理抗性影响。[结果]与未接种处理相比,接种处理抑制100 mg/kg Cu、300 mg/kg Pb处理下POD、CAT以及SOD活性,但是显著提高可溶性蛋白含量。200 mg/kg Cu处理下,接种处理抑制SOD活性却提高POD、CAT活性和可溶性蛋白含量。600 mg/kg Pb处理接种处理下SOD、POD活性与未接种处理无显著区别,但是显著抑制CAT活性以及降低可溶性蛋白含量。以上结果说明,在不同的Cu、Pb浓度处理下,丛枝菌根真菌对旱稻体内的酶活性以及可溶性蛋白影响不同,因而对于重金属引起的旱稻地上部分氧化胁迫的影响程度不同。[结论]该研究可为土壤重金属污染的生物修复提供理论参考。
[Objective] The research aimed to study the effects of arbuscular mycorrhizal fungi on the oxidative stress of upland rice under Cu and Pb treatments. [Method] Pot experiment was conducted to study the effect of arbuscular mycorrhizal fungi on the Cu concentration (0, 100, 200 mg / kg), Pb concentration (0, 300 and 600 mg / kg) Physiological resistance of upland rice under treatment. [Result] Compared with the non-inoculation treatment, inoculation inhibited the activities of POD, CAT and SOD under 100 mg / kg Cu and 300 mg / kg Pb, but significantly increased the content of soluble protein. Under the treatment of 200 mg / kg Cu, the inoculation inhibited the activity of SOD and increased the activity of POD and CAT and the content of soluble protein. Under the inoculation of 600 mg / kg Pb, the activities of SOD and POD were not significantly different from those of non-inoculation, but significantly inhibited the activity of CAT and the content of soluble protein. The above results show that under the different concentrations of Cu and Pb, arbuscular mycorrhizal fungi have different effects on enzyme activities and soluble proteins in upland rice, and therefore have different degrees of influence on the partial oxidation of upland rice caused by heavy metals. [Conclusion] The study could provide theoretical reference for the bioremediation of soil heavy metal pollution.