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以巢式PCR扩增急性淋巴细胞白血病患儿骨髓细胞中,T细胞受体基因V82-D83片段。受试病例有67%(41/61例)获约80~89hP的DNA片段。提示V82-D83基因重排亚型是具有一定代表的类型,对于鉴定白血病残留克隆,具有一定的临床价值。
In nested PCR amplification of bone marrow cells from children with acute lymphoblastic leukemia, T cell receptor gene V82-D83 fragment. 67% of the cases tested (41/61 cases) had a DNA fragment of about 80-89 hP. Tip V82-D83 gene rearrangement subtypes is a representative of the type, for the identification of leukemia residual clones, has a certain clinical value.