[Objective] The aim of this study is to quantitatively analyze the Fusarium concentration and distribution in air and the surrounding substrates (feed, feces, dust, soil) in the chicken house to provide a scientific basis for the control and early warning of Fusarium infection in poultry and practition-ers. [Method] Air samples collected by a 6-stage Anderson air microorganism sampler and exposed culture plates as well as substrate samples gathered by aseptic bags were cultured, counted and purified in the lab. [Result] 717 strains of Fusarium isolates were obtained and the dust had the highest Fusarium concentration among the samples (2.8×105 - 1.5×106 CFU/g with an average of 8.8 x l0s CFU/g). [Conclusion] The Fusarium distribu-tion in the air was in high correlation with those in soil and dust.