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目的探讨移植肾急性排斥（ＡＲ）时细胞凋亡与Ｆａｓ和Ｆａｓ配体（ＦａｓＬ）表达的作用及其临床意义。方法分别用原位末端标记技术（ＴＵＮＥＬ法）和免疫组织化学方法检测２６例移植肾ＡＲ标本中细胞凋亡和Ｆａｓ／ＦａｓＬ表达情况。结果细胞凋亡和Ｆａｓ／ＦａｓＬ表达主要在ＡＲ移植肾小管上皮发生，且凋亡指数和Ｆａｓ／ＦａｓＬ表达与肾组织病理损伤程度平行，与正常肾对照组和移植肾功能稳定组比较差异显著（Ｐ＜０．０１）。结论肾小管上皮细胞凋亡在ＡＲ所致的移植肾损伤中起重要作用，Ｆａｓ／ＦａｓＬ系统可能参与移植肾ＡＲ，是造成肾小管上皮细胞凋亡的重要因素。ＴＵＮＥＬ法检测细胞凋亡可作为判断移植肾病理变化和预后的重要指标
Objective To investigate the role of apoptosis and the expression of Fas and Fas ligand (FasL) in acute rejection of renal allograft (AR) and its clinical significance. Methods The apoptosis and the expression of Fas / FasL in 26 AR specimens of renal allografts were detected by TUNEL and immunohistochemistry respectively. Results Apoptosis and Fas / FasL expression mainly occurred in the renal tubule epithelium of AR transplantation. The apoptosis index and the expression of Fas / FasL were paralleled with the degree of pathological damage in renal tissue, and were significantly different from those in normal renal transplantation group and stable renal graft group P <0.01). Conclusion Apoptosis of renal tubular epithelial cells plays an important role in renal injury induced by AR. Fas / FasL system may play an important role in renal tubular epithelial cell apoptosis. TUNEL assay of apoptosis can be used as an important indicator of pathological changes and prognosis of renal grafts