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目的:研究力竭运动及恢复期间大鼠骨骼肌及心肌肌肉生长抑制素(MSTN)、转化生长因子β(TGF-β)和血小板生长因子(PDGF)的变化。方法:Wistar雄性大鼠24只,随机分为4组,每组6只。E0组、E24组、E48组均进行一次性跑台力竭运动,并分别于力竭即刻、24小时后、48小时后处死;对照组(C组)不进行运动干预,随运动组后处死。取心肌与左侧腓肠肌,ELISA检测组织中MSTN、TGF-β和PDGF。结果:一次力竭运动后,与C组相比,E0组、E24组、E48组骨骼肌和心肌中MSTN、TGF-β的含量均显著性下降(P<0.05,P<0.01),PDGF含量则显著性升高(P<0.01);E0、E24、E48组间无显著性差异。结论:MSTN、TGF-β可能在骨骼肌、心肌修复及肌卫星细胞激活中起负调节作用,PDGF可能在骨骼肌、心肌修复中起正调节作用。
Objective: To investigate the changes of myostatin and transforming growth factor β (TGF-β) and platelet-derived growth factor (PDGF) in skeletal muscle and myocardium during exhaustive exercise and recovery. Methods: Twenty-four Wistar male rats were randomly divided into 4 groups with 6 rats in each group. E0 group, E24 group and E48 group all performed one-time treadmill exhaustion exercise, and were exhausted immediately, 24 hours later and 48 hours later. The control group (C group) . Myocardium and left gastrocnemius muscle were harvested and MSTN, TGF-β and PDGF in the tissue were detected by ELISA. Results: Compared with group C, the levels of MSTN and TGF-β in skeletal muscle and myocardium of E0 group, E24 group and E48 group were significantly decreased (P <0.05, P <0.01) (P <0.01). There was no significant difference between E0, E24 and E48 groups. CONCLUSION: MSTN and TGF-β may play a negative regulatory role in skeletal muscle, myocardial repair and muscle satellite cell activation. PDGF may play a positive regulatory role in skeletal muscle and myocardial repair.