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目的证实在非甲-非庚型病毒性肝炎患者肝组织中一种新型肝炎病毒（transfusion－transmittedvirus，TTV）的存在．方法采用地高辛素标记TTVDNA探针以原位杂交技术对51例血清学病毒标记非甲-非戊型、免疫组化检测肝组织中HGVNSS阴性的病毒性肝炎患者石蜡包埋肝组织进行了检测．结果各型病毒性肝炎肝组织中TTV基因的总检出率为27．5％，其中急性轻型肝炎的检出率为30．8％（4／13），急性重型肝炎（1／8，12．5％），亚急性重型肝炎（3／7，42．9％），慢性肝炎（2／6，33．3％），活动性肝硬变（2／9，22．2％），慢性重型肝炎（1／4，25％），原发性肝癌（1／4，25％）TTVDNA表达于肝细胞核或胞浆内，以核型多见．在急性肝炎，TTV阳性细胞弥漫分布于肝小叶内，慢性肝炎于汇管区附近较为密集，而在肝硬变病例，阳性细胞在假小叶内多呈片簇状不规则分布结论在不明原因病毒性肝炎患者血清及肝组织中TTVDNA的检出表明TTV为一种新型的肝炎病毒，TTV为一种嗜肝性病毒，在我国存在着TTV感染
Objective To confirm the presence of a novel transfusion-transmitted virus (TTV) in the liver tissue of non-A-non-G hepatitis patients. Methods Totally 51 cases of serological virus labeled non-A-non-E were detected by digoxigenin-labeled TTVDNA probe by in situ hybridization. Immunohistochemistry was used to detect the presence of HGVNSS-negative viral hepatitis in the liver of patients with paraffin-embedded liver tissue Detection. Results The overall detection rate of TTV gene was 27.5% in liver tissues of various types of viral hepatitis. The detection rate of acute hepatitis was 30.8% (4/13), acute severe hepatitis (1/8, 12 Subacute severe hepatitis (3/7, 42.9%), chronic hepatitis (2/6, 33.3%), active cirrhosis (2/9, 22.2%), chronic Severe hepatitis (1/4, 25%), primary liver cancer (1/4, 25%) TTVDNA expression in the nucleus or cytoplasm of liver cells, mostly karyotype. In acute hepatitis, TTV positive cells are diffusely distributed in the hepatic lobule, chronic hepatitis is more dense in the vicinity of the portal area, while in the cirrhotic cases, the positive cells are irregularly distributed in the cluster within the false lobule. Conclusion In unexplained viral Detection of TTVDNA in serum and liver tissue of patients with hepatitis shows that TTV is a new type of hepatitis virus and TTV is a hepadnavirus. TTV infection exists in our country