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探讨在临床常规检测条件下 ,混合血清法检测丙型肝炎抗体的效果。方法 :31 5例病人标本分 7次 (每次 45人 )进行检测 ,并将单个血清标本与混合血清标本放在同一块检测板上进行。在单个血清检测结果未知的条件下 ,按自然顺序将 5个血清混合 ,使用酶联免疫法检测丙型肝炎抗体。在检测中保持混合血清中每个标本的稀释度与单个血清检测法相同。结果 :此次抗 - HCV检测的阳性率为 9.2 % ,以单个血清检测为对照 ,混合血清法的特异度为 99.7% ,灵敏度为 89.7% ;Kappa值为 0 .92 (P<0 .0 5)。收益分析发现 ,应用混合血清法检测抗 - HCV可获得显著的经济效益。结论 :作者认为 ,临床在用 EIA法检测抗 - HCV时 ,将 5个标本混合进行检测是可行的。
To investigate the effect of mixed sera assay on hepatitis C antibody in clinical routine testing. Methods: 31 5 specimens of patients were divided into 7 times (45 each time) for testing, and a single serum sample and mixed serum samples were placed on the same test board. In a single serum test results unknown conditions, in accordance with the natural sequence of 5 sera were mixed, the use of enzyme-linked immunosorbent assay for hepatitis C antibodies. The dilution of each specimen in the pooled serum during the assay is the same as the single serum assay. Results: The positive rate of this anti-HCV test was 9.2%. The single serum test was used as the control. The specificity of mixed serum test was 99.7%, the sensitivity was 89.7%, the Kappa value was 0.92 (P <0.05) ). Benefit analysis found that the application of mixed serum detection of anti-HCV can be significant economic benefits. Conclusion: The authors believe that it is practicable to test the combination of 5 specimens for detection of anti-HCV by EIA in clinic.