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目的探讨DD3基因不同外显子在前列腺癌组织中的特异性表达。方法根据基因库所收录的DD3 mRNA序列,设计跨越不同外显子的3对DD3 mRNA序列特异的引物,应用逆转录-聚合酶链反应(RT-PCR)对61例前列腺组织和70例前列腺外的其他肿瘤组织及癌旁组织进行DD3 mRNA检测,并对前列腺癌组织DD3 mRNA进行全序列分析。结果跨越DD3 mRNA外显子1与3、外显子1与4及外显子3与4之间设计的引物所检测DD3 mRNA结果完全一致,21例前列腺癌组织与1例前列腺上皮内肿瘤组织中DD3 mRNA均为阳性,39例良性前列腺增生组织和70例其他肿瘤组织及癌旁组织中D133 mRNA均为阴性。21例前列腺癌组织DD3 mRNA序列之间完全一致(GenBank登陆号为AY894120),与国外报道序列(AF103907)之间有99．8%同源。结论DD3 mRNA仅在前列腺癌中特异性表达,外显子3和外显子4均可作为DD3 mRNA特异的外显子。
Objective To investigate the specific expression of different exons of DD3 gene in prostate cancer tissues. Methods According to the DD3 mRNA sequence of gene library, three pairs of primers specific to DD3 mRNA that spanned different exons were designed. Reverse transcription polymerase chain reaction (RT-PCR) was performed on 61 cases of prostate and 70 cases of extra-prostatic DD3 mRNA was detected in other tumor tissues and paracancerous tissues, and the full-length sequence analysis of DD3 mRNA in prostate cancer tissue was performed. Results The results of DD3 mRNA detected by primers designed between exon 1 and exon 1 and exon 1 and exon 3 and 4 across DD3 mRNA were identical with that of DD3 mRNA detected in 21 cases of prostate cancer and 1 case of prostatic intraepithelial neoplasia DD3 mRNA were all positive, 39 cases of benign prostatic hyperplasia tissue and 70 cases of other tumor tissues and adjacent tissues D133 mRNA were negative. The sequence of DD3 mRNA in 21 cases of prostate cancer was completely identical (GenBank accession No. AY894120), and 99.8% homologous with the foreign reporter sequence (AF103907). Conclusion DD3 mRNA is only expressed specifically in prostate cancer. Both exon 3 and exon 4 are specific exons of DD3 mRNA.