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淋巴细胞转化(淋转)为细胞免疫功能测定指标之一。1981年世界卫生组织建议,测定有丝分裂原对淋巴细胞的增殖反应,最好采用放射性同位素标记的胸腺嘧啶核苷掺入法。本文对该法进行了探索。试验材料一、标本:采自健康输血员81例,每人取新鲜肝素抗凝血。二、营养液:用RPMI—1640培养液,加10％小牛血清、青霉素100u/ml、链霉素100μg/1ml。三、有丝分裂原:植物血凝素(PHA,广州市广东医学工业研究所产品)。工作浓度1000μg/1ml。四、氚胸腺嘧啶核甘(~3H-TDR,北京原子能研究所供给)用生理盐水稀释成20μci/1mg,4C保存备用。
Lymphocyte transformation (lymph node metastasis) as one of the indicators of cellular immune function. The World Health Organization recommended in 1981 to measure the proliferative response of mitogen to lymphocytes, preferably using radioisotope-labeled thymidine incorporation. This article explores the law. Test Materials First, specimens: collected from 81 healthy blood transfusion, each taking fresh heparin anticoagulant. Second, nutrient solution: RPMI-1640 medium, add 10% calf serum, penicillin 100u / ml, streptomycin 100μg / 1ml. Third, mitogen: phytohemagglutinin (PHA, Guangzhou, Guangdong Institute of Medical Industry Products). Working concentration 1000μg / 1ml. Fourth, tritium thymidine (~ 3H-TDR, Beijing Institute of Atomic Energy supplied) diluted with saline into 20μci / 1mg, 4C for later use.