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目的：制备并纯化小鼠黑色素凝集素（MMA）。方法：利用亲合层析及凝胶过滤层桥从小鼠B16黑色素瘤中分高纯化小鼠黑色素瘤凝集素（MMA）。结果：利用亲和层材制备的凝集素，经SDS-PAGE显示出一条分子量12．4kD的主要蛋白带和两个分子量为35kD和67kD的痕迹蛋白带；经凝胶过滤层析则出现分子量为26kD主峰及分子量为35kD和67kD的两个小峰。26kD的蛋白成分经SDS－PAGE和等电聚焦电泳显示出一条均一的蛋白带。26kD的蛋白成分特异性识别会多聚乙酰氢基乳精的糖链，活性依赖于巯基而非钙离子。结论：26kD的成分为MMA，利用经亲和层析和凝胶过滤层析等可得到成分均一的MMA，MMA为S型凝集素。
Objective: To prepare and purify mouse melanin lectin (MMA). METHODS: Mouse melanoma lectin (MMA) was purified from mouse B16 melanoma using affinity chromatography and gel filtration bridges. RESULTS: The lectin prepared by the affinity layer material showed a main protein band with a molecular weight of 12.4 kD and two trace protein bands with molecular weights of 35 kD and 67 kD by SDS-PAGE. The molecular weight was found by gel filtration chromatography. The 26kD main peak and two small peaks with molecular weights of 35kD and 67kD. The protein component of 26 kD showed a uniform protein band by SDS-PAGE and isoelectric focusing. The 26 kD protein component specifically recognizes the poly(glycosyl) lactate sugar chain, and the activity is dependent on the thiol group rather than the calcium ion. Conclusion: The component of 26kD is MMA. MMA with homogeneous composition can be obtained by affinity chromatography and gel filtration chromatography. MMA is S-type lectin.