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强氯精是水产养殖过程中普遍使用的消毒剂,为了解强氯精能够杀菌却不会对养殖水产动物尤其是其繁殖功能产生危害的有效浓度,以分离自尼罗罗非鱼(Oreochromis niloticus)精巢的N-乙酰-β-D-氨基葡萄糖苷酶(EC3.2.1.52,NAGase)为材料,利用底物反应的动力学方法探讨强氯精对该酶的失活动力学.结果显示该酶在强氯精溶液中是一个可逆的反竞争型的失活过程,其对酶抑制的IC50为(0.25±0.02)mg/m L;而且强氯精能降低该酶的热稳定性和p H稳定性;通过建立失活模型,计算得知正向和逆向微观失活速度常数k′+0和k′-0值分别为3.84×10~(-3)/s和8.37×10~(-4)/s.本研究结果可为评价强氯精对罗非鱼繁殖功能的影响提供理论依据.
Strong Chlorine is a commonly used disinfectant in aquaculture. In order to understand the effective concentration of Strong Chlorine that can be sterilized without harming cultured aquaculture animals, and in particular their reproductive function, strong isolates of Oreochromis niloticus ) Testis N-acetyl-β-D-glucosaminidase (EC3.2.1.52, NAGase) as the material, the use of substrate reaction kinetics method of strong chlorine on the enzymatic inactivation kinetics of the enzyme results show that the The enzyme is a reversible anti-competitive inactivation process in strong chlorogenic acid solution with an IC50 of (0.25 ± 0.02) mg / m L for enzyme inhibition; and strong chlorine can reduce the thermostability of the enzyme and p H and the stability constants of k’-0 and k’-0 were calculated by setting up inactivation model. The calculated values of k ’+ 0 and k’-0 for the inversed and the reverse microscopic inactivation rates were 3.84 × 10 -3 / s and 8.37 × 10 ~ 4) / s.The results of this study may provide a theoretical basis for evaluating the effect of strong chlorine on the reproductive function of tilapia.