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分别以一定浓度及一定数量的人表皮细胞进行培养,其融合成片的时间及OD值行MTT检测,结果,rhEGF的最佳浓度为10μg/L,100μg/L时起抑制作用,提示适宜浓度rhEGF可加速上皮细胞增长,对烧伤临床应用及其基础研究具潜在价值。
Respectively, a certain concentration and a certain number of human epidermal cells were cultured, the time and OD value of the fusion into slices MTT assay, the results, rhEGF optimal concentration of 10μg / L, 100μg / L when the inhibition, suggesting that the appropriate concentration rhEGF accelerates the growth of epithelial cells and has potential value in the clinical application of burns and its basic research.