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以体外培养的PTK_2和HeLa细胞株为试验材料,用氦氖激光显微仪照射摄入HPD的单细胞,以细胞出现初级可见损伤时间为指标,对国产HPD和美国的Photofrin-Ⅱ的药效作了分析比较。讨论了把这一方法应用于临床前研究的可能性。
Using PTK_2 and HeLa cells cultured in vitro as experimental materials, HPD-injected single cells were irradiated with holmium laser microscopy. The initial visible damage time of cells was used as an index to measure the efficacy of domestically produced HPD and American Photofrin-II. Analyzed and compared. The possibility of applying this method to preclinical studies was discussed.