随着生命科学的发展和疾病诊断要求的不断提高,高灵敏度、高选择性、快速简便的分析方法越来越受到重视.基于新原理发展用于生物分子检测的新技术和新方法,已经成为现代分析化学研究的主题和热点,对国家经济社会的可持续发展具有重要意义.分子荧光探针具有灵敏度高、操作简便、重现性和膜穿透性好等优点.此外,与荧光成像技术相结合,分子荧光探针适用于生物体系中目标分子的实时原位无损检测,从而实现活细胞与活体中生物分子及其生物过 With the development of life sciences and the increasing demand for disease diagnosis, more and more attention has been paid to high sensitivity, high selectivity, and rapid and simple analysis methods. The development of new techniques and new methods for biomolecular detection based on new principles has become The theme and hot spots of modern analytical chemistry research are of great significance for the sustainable development of the national economy and society.Molecular fluorescence probes have the advantages of high sensitivity, simple operation, reproducibility and good membrane penetration.In addition, with the fluorescence imaging technology In combination, the molecular fluorescent probe is suitable for real-time in situ non-destructive detection of target molecules in biological systems, thus enabling biomolecules and their biological activities in living cells and living organisms