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单链构象多态性（SSCP）分析已成为检测基因突变的重要手段。结合PCR技术对肿瘤组织抑癌基因Rb的存在状态进行检测，具有重要的临床基因诊断意义。该研究主要建立了PCR－SSCP—银染方法，并对获得SSCP最佳图型的实验条件进行了研究。通过PCR对Rb基因14．27两个外显子分别进行扩增，得到212和218bP的DNA片断，加热变性后，在含5％甘油的8％非变性聚丙烯酰胺凝胶（acr：bis＝49：1）以0．5×TBE，冷房中恒功率电泳，以使凝胶温度分别恒定在16℃和20℃，即可得到稳定的SSCP分析结果。
Single-stranded conformation polymorphism (SSCP) analysis has become an important method for detecting gene mutations. Combined with PCR technology to detect the existence of tumor suppressor gene Rb, has important clinical diagnostic significance. The study mainly established the PCR-SSCP-silver staining method and studied the experimental conditions for obtaining the best SSCP pattern. The exons of the Rb gene 14.27 were amplified by PCR to obtain 212 and 218 bP DNA fragments, which were denatured by heat and then 8% non-denatured polyacrylamide gel containing 5% glycerol (acr:bis= 49:1) A constant SSCP analysis result can be obtained by constant power electrophoresis in a cold room at 0.5×TBE so that the gelation temperatures are constant at 16° C. and 20° C. respectively.