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目的:探讨常规方法(冲击量+维持量)补充肌酸对大鼠自身肌酸生物合成代谢的影响。方法:冲击剂量(1.5g/kg/d)补充肌酸1周和维持剂量(0.15g/kg/d)补充肌酸1月、2月后,分别测定大鼠肾脏精氨酸-甘氨酸脒基转移酶(AGAT)活性,肾脏和肝脏胍乙酸含量,肾脏、骨骼肌和血清肌酸含量,血清和骨骼肌肌酸激酶(CK)活性及血清肌酐水平。结果:冲击量及维持量补充肌酸均可导致大鼠肾脏AGAT活性以及肾脏和肝脏胍乙酸含量降低,引起肾脏肌酸含量明显增加,肾脏和血清肌酸含量与AGAT酶活性呈明显负相关。冲击期血清肌酐水平显著升高,但在维持期恢复正常。结论:常规方法(冲击量+维持量)补充肌酸可显著抑制内源性肌酸的合成。
OBJECTIVE: To investigate the effects of creatine supplementation on creatine biosynthesis and metabolism in rats by conventional methods (impact + maintenance). Methods Creatine was supplemented with 1.5g / kg / d creatine for 1 week and maintenance dose (0.15g / kg / d) for 1 month and 2 months respectively. The levels of arginine - glycine amidinate AGAT activity, guanidinoic acid content in kidney and liver, creatine content in kidney, skeletal muscle and serum, serum and skeletal muscle creatine kinase (CK) activity and serum creatinine. RESULTS: Both creatine and creatine levels in the kidneys and liver were decreased after the shock and maintenance creatinine supplementation. Creatine and creatine levels in the kidneys and serum were significantly negatively correlated with AGAT activity. The impact of serum creatinine levels were significantly increased, but returned to normal in the maintenance phase. CONCLUSIONS: Creatine supplementation can significantly inhibit the synthesis of endogenous creatine by conventional methods (impact + maintenance).