人白细胞介素12诱生表达及双启动子真核表达载体的构建

来源 :中国肿瘤生物治疗杂志 | 被引量 : 0次 | 上传用户:dlj0425jack
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白细胞介素12(IL-12)主要是由抗原提呈细胞产生的一种异二聚体(P40和P35)细胞因子,能显著增强NK/LAK以细胞的杀伤活性,促进特异性CTL细胞的应答能力,诱导T细胞和NK细胞大量分泌IEN-γ,启动Tho细胞向Thl细胞的发育,在抗肿瘤、爱滋等多种疾病中将发挥重要作用.我们用SAC,LPS,IFN-γ和PDBu等刺激剂作用外周血单个核细胞或口腔表皮样癌细胞株KB,成功诱生出IL-12-p40和p35,并插 Interleukin-12 (IL-12) is a heterodimeric (P40 and P35) cytokine mainly produced by antigen-presenting cells and can significantly enhance NK / LAK cell killing activity and promote specific CTL cell Response ability to induce large numbers of T cells and NK cells to secrete IEN-γ, and to initiate the development of Tho cells to Th1 cells, which play an important role in many diseases such as cancer and AIDS.We used SAC, LPS, IFN-γ and PDBu stimulation of peripheral blood mononuclear cells or oral epidermoid carcinoma cell line KB, successfully induced IL-12-p40 and p35, and inserted
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