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目的:通过对牡荆素葡萄糖苷单体在小鼠体内的吸收、分布、排泄的研究,为其在动物体内全面的药代动力学的探索增加科学依据。方法:采用3p97药代动力学软件处理由液相色谱得到的数据。结果:小鼠口服牡荆素葡萄糖苷后的吸收过程是迅速的,在胃肠道的环境下处于一个弱吸收,在小鼠体内存在肝肠循环,还经历了较大程度的首过效应,被代谢成其它化合物。结论:建立了两种测定小鼠生物样品中牡荆素葡萄糖苷的高效液相色谱方法,一种用于血浆和组织样品,另一种用于排泄物尿液和粪便。两种方法都具有高度的专属性和可重复性。得到的实验数据真实可靠,填补了山楂叶中牡荆素葡萄糖苷的临床前药代动力学研究的空缺。
OBJECTIVE: To investigate the absorption, distribution and excretion of glucosidic monomers of vitexin in mice and provide scientific basis for its comprehensive pharmacokinetic exploration in animals. Methods: The data obtained from liquid chromatography were processed using the 3p97 pharmacokinetic software. Results: The absorption of vitexin glucoside was rapid in mice and was weakly absorbed in the environment of gastrointestinal tract. There was enterohepatic circulation in mice and also experienced a large first pass effect. Metabolism into other compounds. Conclusions: Two HPLC methods for the determination of vitexin glucosides in mouse biological samples have been established, one for plasma and tissue samples and one for excrement urine and feces. Both methods are highly specific and repeatable. The experimental data obtained are true and reliable, filling vacancies in preclinical pharmacokinetic studies of vitexin glucoside in hawthorn leaves.