本文报告1例在临床诊断胰腺癌三年半前从收集的纯净胰腺液中检出第12位密码子突变的K－ras基因。患者男性，对岁，其胰腺癌系经内镜下逆行胰胆管造影（ERCP）诊断，并示胰腺体尾交界区的主胰管阻塞。行远端胰腺、脾切除术并确诊为胰尾进展性胰腺癌且肝内有少量转移性结节。经回 This article reports a case of a K-ras mutation at the 12th codon that was detected in a pool of pure pancreatic juice collected three and a half years before clinical diagnosis of pancreatic cancer. Male and female patients were diagnosed as having pancreatic cancer diagnosed by endoscopic retrograde cholangiopancreatography (ERCP) and showing obstruction of the main pancreatic duct at the junction of pancreatic body and tail. The distal pancreatic and splenectomy were performed and pancreatic tail progressive pancreatic cancer was diagnosed with a small amount of metastatic nodules in the liver. After