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目的探讨Peyer小结在IgA肾病(IgAN)发病中的作用。方法雄性BALB/C小鼠予牛血清清蛋白、葡萄球菌肠毒素B诱导IgAN模型,另选40只正常雄性BALB/C小鼠作为对照组。第12周免疫荧光检测其肾组织IgA沉积,测定其血清总蛋白(TP)、清蛋白(Alb)、BUN、Cr、半胱氨酸蛋白酶抑制剂C(Cys C)。荧光定量反转录(RT)-PCR检测Peyer小结IL-21、转化生长因子-β1(TGF-β1)mRNA的表达。Western blot测定Peyer小结IL-21、B细胞淋巴瘤因子-6(Bcl-6)、B淋巴细胞诱导成熟蛋白-1(Blimp-1)蛋白表达。结果 1.IgAN组和对照组第0周、第6周、第12周24 h尿蛋白定量分别为(0.61±0.26)mg vs(0.67±0.22)mg、(3.61±0.75)mg vs(0.77±0.27)mg、(4.15±0.82)mg vs(2.09±0.51)mg,IgAN组第6周、第12周24 h尿蛋白均高于对照组(Pa<0.01)。2.IgAN组和对照组IL-21、TGF-β1 mRNA表达相对值分别为1.67±0.13 vs 1.48±0.13、1.21±0.09 vs 1.10±0.10,与对照组相比,IgAN组IL-21、TGF-β1 mRNA表达均增加,差异均有统计学意义(Pa<0.05)。3.Western blot显示IgAN组和对照组IL-21、Bcl-6、Blimp-1蛋白表达相对值分别为0.67±0.21 vs 0.45±0.10、0.34±0.21 vs 0.60±0.19、1.03±0.07 vs 0.67±0.07,与对照组相比,IgAN组IL-21、Blimp-1蛋白表达均显著增高,差异均有统计学意义(Pa<0.05),Bcl-6蛋白表达降低,差异有统计学意义(P<0.05)。结论 Peyer小结有促进B淋巴细胞向分泌IgA浆母细胞分化的微环境,参与IgAN的发病。
Objective To investigate the role of Peyer’s nodules in the pathogenesis of IgA nephropathy (IgAN). Methods Male BALB / C mice were induced with bovine serum albumin (BSA) and staphylococcal enterotoxin B (IgAN) and 40 normal male BALB / C mice were selected as the control group. At the 12th week, IgA deposition in renal tissues was detected by immunofluorescence assay. Total protein (TP), albumin (ALB), BUN, Cr and Cys C were determined by immunofluorescence. Fluorescence quantitative reverse transcription (RT) -PCR was used to detect the expression of IL-21 and transforming growth factor-β1 (TGF-β1) mRNA in Peyer’s nodules. Western blot was used to detect the protein expression of Peyer’s nodule IL-21, B-cell lymphoma factor-6 (Bcl-6) and B lymphocyte-induced mature protein-1 (Blimp-1) Results 1. The protein expressions of 24 h urine in IgAN group and control group at week 0, 6, 12 were (0.61 ± 0.26) mg vs (0.67 ± 0.22) mg, (3.61 ± 0.75) mg vs (0.77 ± 0.27) mg and (4.15 ± 0.82) mg vs (2.09 ± 0.51) mg respectively. Urine protein in 24th and 12th week of IgAN group was higher than that of control group (Pa0.01). The relative expression of IL-21 and TGF-β1 mRNA in IgAN group and control group were 1.67 ± 0.13 vs 1.48 ± 0.13 and 1.21 ± 0.09 vs 1.10 ± 0.10, respectively. Compared with the control group, IL-21 and TGF- β1 mRNA expression were increased, the differences were statistically significant (Pa <0.05). Western blot showed that the relative expression of IL-21, Bcl-6 and Blimp-1 in IgAN group and control group were 0.67 ± 0.21 vs 0.45 ± 0.10,0.34 ± 0.21 vs 0.60 ± 0.19, 1.03 ± 0.07 vs 0.67 ± 0.07 Compared with the control group, the expressions of IL-21 and Blimp-1 in IgAN group were significantly increased (P <0.05), while the expression of Bcl-6 protein in IgAN group was significantly lower (P <0.05 ). Conclusion Peyer’s summary has the microenvironment that promotes the differentiation of B lymphocytes into IgA-producing plasma cells and is involved in the pathogenesis of IgAN.